Doxorubicin HCL
doxorubicin hydrochloride
CAS: 25316-40-9
Molecular Formula: C27H30ClNO11
Doxorubicin HCL - Names and Identifiers
| Name | doxorubicin hydrochloride |
| Synonyms | ADRIACIN ADRIBLASTINA ADRIAMYCIN HCL Doxorubicin HCL Doxorubicin, HCl ADRIAMYCIN HYDROCHLORIDE 14-HYDROXYDAUNOMYCIN HCL doxorubicin hydrochloride ADRIBLASTINA HYDROCHLORIDE 14-HYDROXYDAUNOMYCIN HYDROCHLORIDE (8s-cis)-10-((3-amino-2,3,6-trideoxy-alpha-l-lyxo-hexopyranosyl)oxy)-7,8,9,10-tetrahydro-6,8,11-trihydroxy-8-(hydroxyacetyl)-1-methoxynaphthacene-5,12-dione hydrochloride (8s-cis)-10-[(3-amino-2,3,6-trideoxy-alpha-l-lyxo-hexopyranosyl)oxy]-7,8,9,10-tetrahydro-6,8,11-trihydroxy-8-(hydroxyacetyl)-1-methoxynaphthacene-5,12-dione hydrochloride |
| CAS | 25316-40-9 |
| EINECS | 246-818-3 |
| InChI | InChI=1/C27H29NO11.ClH/c1-10-22(31)13(28)6-17(38-10)39-15-8-27(36,16(30)9-29)7-12-19(15)26(35)21-20(24(12)33)23(32)11-4-3-5-14(37-2)18(11)25(21)34;/h3-5,10,13,15,17,22,29,31,33,35-36H,6-9,28H2,1-2H3;1H/t10-,13-,15-,17-,22+,27-;/m0./s1 |
| InChIKey | MWWSFMDVAYGXBV-RUELKSSGSA-N |
Doxorubicin HCL - Physico-chemical Properties
| Molecular Formula | C27H30ClNO11 |
| Molar Mass | 579.98 |
| Melting Point | 216°C (dec.)(lit.) |
| Boling Point | 810.3°C at 760 mmHg |
| Specific Rotation(α) | D20 +248±2° (c = 0.1 in methanol) |
| Flash Point | 443.8°C |
| Water Solubility | Soluble in water. |
| Solubility | Easily soluble in water and methanol, slightly soluble in ethanol, very slightly soluble in chloroform, almost insoluble in acetone. |
| Vapor Presure | 9.64E-28mmHg at 25°C |
| Appearance | Orange-Red Powder |
| Color | orange to dark red |
| Maximum wavelength(λmax) | ['497nm(H2O)(lit.)'] |
| Merck | 14,3439 |
| BRN | 4229251 |
| pKa | pKa 8.25±0.60 (Uncertain);8.43±0.70 (Uncertain);11.9±0.4 (Uncertain);12.95±0.1 (Uncertain);13.8±0.70 |
| Storage Condition | 2-8°C |
| Stability | Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20° for up to 3 months. |
| MDL | MFCD04974507 |
| Physical and Chemical Properties | Hydrochloride is an orange-red needle-like crystal with a melting point of 204-205 °c. Soluble in water and alcohol, insoluble in acetone, benzene, chloroform, ether. |
Doxorubicin HCL - Risk and Safety
| Risk Codes | R45 - May cause cancer R22 - Harmful if swallowed R40 - Limited evidence of a carcinogenic effect R26/27/28 - Very toxic by inhalation, in contact with skin and if swallowed. R36/38 - Irritating to eyes and skin. |
| Safety Description | S53 - Avoid exposure - obtain special instructions before use. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S22 - Do not breathe dust. S7/9 - |
| WGK Germany | 3 |
| RTECS | QI9295900 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 29419090 |
| Toxicity | LD50 i.v. in mice: 21.1 mg/kg (Bertazzoli, 1970) |
Doxorubicin HCL - Standard
Authoritative Data Verified Data
This product is (8S,10s)-10-[(3-amino -2,3, 6-tridesoxy-a -L-lysohexopyranyl)-Oxygen]-7,8,9, 10-tetrahydro-6, 8, 11-trihydroxy-8-(hydroxyacetyl) -1-methoxy-5, 12-naphthalene Dione hydrochloride. Doxorubicin hydrochloride (C27H29NO11 • HCl) should be 98.0% to 102.0% based on water and solvent-free material.
Last Update：2024-01-02 23:10:35
Doxorubicin HCL - Trait
Authoritative Data Verified Data
- This product is orange-red crystalline powder; With hygroscopicity.
- This product is dissolved in water and slightly soluble in methanol.
Last Update：2022-01-01 15:03:51
Doxorubicin HCL - Introduction
DaunorubiCln HCl inhibits DNA and RNA synthesis, Ki is 0 · 02 μM when inhibiting DNA synthesis. Powerful anticancer agents have been shown to be strong inhibitors of DNA and RNA synthesis. Autofluorescence anthracycline antibiotics, anticancer drugs. Substrates of Pgp, MRP-1 and BCRP. Fluorescence properties have been used to measure drug efflux pump activity, as well as to solve many important problems, such as the intracellular localization of multiple multidrug-resistant proteins and the role of suborganelles (Golgi apparatus and lysosomes) in drug chelation and their implications in drug-resistant phenotypes. Daunorubicin is chelated into lysosomes according to a pH-partitioning mechanism. Strong inhibitors of DNA and RNA synthesis.
Last Update：2022-10-16 17:15:20
Doxorubicin HCL - Differential diagnosis
Authoritative Data Verified Data
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- take this product, add methanol to make a solution containing about 20ug per lml, according to UV-visible spectrophotometry (General 0401), at 233nm, 252nm, 288nm, 478nm, 495nm and 530nm wavelength has the maximum absorption.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 1015).
- Take 10mg of this product, add 0.5ml nitric acid to dissolve, add 0.5ml of water, flame burn for 2 minutes, let it cool, drop silver nitrate test solution to generate white curd-like precipitate.
Last Update：2022-01-01 15:03:52
Doxorubicin HCL - Exam
Authoritative Data Verified Data
crystallinity
take a small amount of this product, according to the law inspection (General 0981), should comply with the provisions.
acidity
take this product, add water to make a solution containing 5mg per lml, according to the law (General 0631) , pH value should be 4.0~5.5.
Related substances
take an appropriate amount of this product, add the mobile phase to dissolve and dilute to make about 1 in each lml. 0 mg of the solution was used as a test solution; An appropriate amount of the solution was quantitatively diluted with a mobile phase to prepare a solution containing about 1 ug per 1 ml as a control solution. According to the chromatographic conditions under the content determination item, 10 u1 of the test solution and 10 u1 of the control solution are accurately weighed and injected into the human liquid chromatograph respectively, and the chromatogram is recorded to 3.5 times of the retention time of the main component peak. If there are impurity peaks in the chromatogram of the test solution, the area of a single impurity peak shall not be greater than 0.5 times (0.5%) of the area of the main peak of the control solution, the sum of each impurity peak area shall not be greater than 2 times (2.0%) of the main peak area of the control solution.
residual solvent
take about 0.2g of this product, precision weighing, set in the top empty bottle, precision add water 5ml to dissolve, seal, as a test solution; Respectively, precision weighing methanol, ethanol, each appropriate amount of acetone and dichloromethane was quantitatively diluted with dimethyl sulfoxide to prepare their respective stock solutions, and each appropriate amount was quantitatively diluted with water to prepare each lml containing 20ug of methanol and 0.2mg of ethanol, the mixed solution of acetone 10ug and dichloromethane 2ug was precisely weighed into 5ml, placed in a headspace bottle, and sealed as a reference solution. According to the test for determination of residual solvents (General rule 0861 first method), the capillary column with 6% cyanopropyl phenyl-94% dimethyl polysiloxane (or similar polarity) as stationary liquid is used as the chromatographic column, and the column temperature is 50°C; the inlet temperature was 140°C; The detector temperature was 250°C; The carrier gas was helium or nitrogen, and the flow rate was 5.0 mL per minute. The Headspace sample was injected, and the equilibrium temperature of the headspace bottle was 90°C, and the equilibrium time was 30 minutes. The reference solution was sampled by Headspace injection, and the chromatogram was recorded. Methanol, ethanol, acetone and dichloromethane peaks in sequence, and the separation degree between the four main peaks should meet the requirements. The test solution and the reference solution were injected into the headspace respectively, and the chromatogram was recorded, and the peak area was calculated according to the external standard method. Ethanol residues should not exceed 1.0%, methanol, acetone and dichloromethane residues should be in accordance with the provisions.
moisture
take this product, according to the moisture determination method (General 0832 first method 1), the water content shall not exceed 4.0%.
bacterial endotoxin
take this product, check according to law (General 1143), each 1 mg doxorubicin hydrochloride containing endotoxin amount should be less than 2.0EU. (For injection)
antihypertensive substances
take this product, check according to law (General rule 1145), the dose of cat weight per lkg injection 1.5mg, should meet the requirements. (For injection)
Last Update：2022-01-01 15:03:53
Doxorubicin HCL - Content determination
Authoritative Data Verified Data
measured by high performance liquid chromatography (General 0512).
chromatographic conditions and system suitability test
silica gel bonded with eighteen alkyl silane as filler; Sodium dodecyl sulfate solution (take sodium dodecyl sulfate 1.44G and phosphoric acid 0.68ml, add water 500ml to dissolve)-acetonitrile-methanol (500:500:60) as the mobile phase; The detection wavelength is 254nm; Take the appropriate amount of doxorubicin hydrochloride reference and epirubicin hydrochloride reference, dissolve and dilute with the mobile phase to make a mixed solution containing 50ug in each lml, take 10u1 injection human liquid chromatograph, record chromatogram, the separation degree between doxorubicin peak and epirubicin peak should be greater than 2.0.
assay
take the appropriate amount of this product, precision weighing, plus mobile phase dissolution and quantitative dilution made from each lml containing about O. 1 mg of the solution, as the test solution, the precise amount of 10u1 was injected into the liquid chromatograph, and the chromatogram was recorded. The control substance of doxorubicin hydrochloride was taken and determined by the same method. The peak area was calculated according to the external standard method.
Last Update：2022-01-01 15:03:54
Doxorubicin HCL - Category
Authoritative Data Verified Data
Anti-tumor antibiotics.
Last Update：2022-01-01 15:03:54
Doxorubicin HCL - Storage
Authoritative Data Verified Data
shade, seal, and store in a cool place.
Last Update：2022-01-01 15:03:54
Doxorubicin HCL - Adriamycin (Doxorubicin Hydrochloride for Injection)
Authoritative Data Verified Data
This product is a sterile freeze-dried product made of doxorubicin hydrochloride plus appropriate amount of lactose or other excipients. Doxorubicin hydrochloride containing (C27H29NO11 • HCl) shall be between 90.0% and 110.0% of the labeled amount.
trait
This product should be orange-red loose lumps or powder.
identification
This product was taken and the same results were shown according to the tests of (1) and (4) under the item of doxorubicin hydrochloride.
examination
- acidity take this product, add water to make a solution containing 5mg of doxorubicin hydrochloride per lml, and determine it according to law (General rule 0631). The pH value should be 4.5~6.5.
- relevant substances: Take appropriate amount of this product, add mobile phase to dissolve and dilute to make doxorubicin hydrochloride 1 per 1 ml. 0 mg solution, as the test solution, according to the method under the item of doxorubicin hydrochloride, doxorubicin ketone (take doxorubicin hydrochloride control about 10 mg, add water 5ml and phosphoric acid 5ml, shake, place it for 30 minutes, adjust the pH value to 2.6 with 2mol/L sodium hydroxide solution, add 15ml of acetonitrile and 10ml of methanol, and mix well to obtain doxorubicin ketone control solution). The Peak area should not be greater than the main peak area of the control solution (1.0% ) ; other single impurity peak area shall not be greater than 0.5 times (0.5%) of the main peak area of the control solution, and the sum of each impurity peak area shall not be greater than 3 times (3.0%) of the main peak area of the control solution. The content uniformity of
- shall be calculated based on the content of each bottle measured under the content determination item, and shall comply with the regulations (General rule 0941).
- moisture, antihypertensive substances and bacterial endotoxin shall be checked according to the method of doxorubicin hydrochloride, and shall be in accordance with the regulations.
- sterile take this product, dissolve and dilute with the appropriate solvent, the membrane filtration method, inspection according to law (General 1101), should comply with the provisions.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
Take 10 bottles of this product, add mobile phase to dissolve and quantitatively dilute according to the indicated amount, and make it into about 0 ml per lml. 1 mg of the solution was measured as a test solution according to the method of doxorubicin hydrochloride, and the average content of 10 vials was determined.
category
Same as doxorubicin hydrochloride.
specification
(l)lOmg (2)50mg
storage
shade, close, and store in a cool place.
Last Update：2022-01-01 15:03:55
Doxorubicin HCL - Daunorubicin Hydrochloride for Injection
Authoritative Data Verified Data
This product is Daunorubicin Hydrochloride and appropriate amount of mannitol or other excipients made of sterile lyophilized. Daunorubicin-containing (C27H29NO10) shall be between 90.0% and 110.0% of the labeled amount.
trait
This product is a red loose block or powder.
identification
take this product, according to Daunorubicin Hydrochloride under the identification of (1), (4) test, showed the same results.
examination
- appropriate amount of related substances of this product, dissolved and diluted with mobile phase to prepare about daunorubicin l per lml. 0 mg of the solution was measured as a test solution according to the method described under Daunorubicin Hydrochloride. The single impurity peak area shall not be more than 2 times (2.0%) the main peak area of the control solution, and the sum of the impurity peak areas shall not be more than 3 times (3.0%) the main peak area of the control solution. The content uniformity of
- shall be calculated based on the content of each bottle measured under the content determination item, and shall comply with the regulations (General rule 0941).
- sterile take this product, dissolve and dilute with appropriate solvent, after the membrane filtration method, inspection according to law (General rule 1101), should comply with the provisions.
- acidity, moisture, bacterial endotoxin and antihypertensive substances shall be checked according to the method under Daunorubicin Hydrochloride, and shall comply with the regulations.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
Take 10 bottles of this product, add mobile phase to dissolve and quantitatively dilute to prepare about 0. 5% daunorubicin per 1 ml. 1 mg of the solution was used as a test solution, and the content of each bottle was measured according to the method of Daunorubicin Hydrochloride, and the average content of 10 bottles was obtained.
category
Same as Daunorubicin Hydrochloride.
specification
20mg (based on C27H29NO10)
storage
shade, close, and store in a cool place.
Last Update：2022-01-01 15:32:53
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Product Name: DOX Visit Supplier Webpage Request for quotationCAS: 25316-40-9
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QQ: 3008007409
Wechat: 18301782025
Multiple SpecificationsSpot supply
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Email: 2853786052@qq.com
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